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human cd3  (Sino Biological)
94
Sino Biological human cd3
KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with <t>CD3</t> bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.
Human Cd3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cd3/product/Sino Biological
Average 94 stars, based on 1 article reviews
human cd3 - by Bioz Stars, 2026-02
94/100 stars
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human cd3 delta  (Sino Biological)
94
Sino Biological human cd3 delta
KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with <t>CD3</t> bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.
Human Cd3 Delta, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cd3 delta/product/Sino Biological
Average 94 stars, based on 1 article reviews
human cd3 delta - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
protein  (Sino Biological)
94
Sino Biological protein
KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with <t>CD3</t> bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.
Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein/product/Sino Biological
Average 94 stars, based on 1 article reviews
protein - by Bioz Stars, 2026-02
94/100 stars
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human cd3 epsilon  (Sino Biological)
94
Sino Biological human cd3 epsilon
KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with <t>CD3</t> bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.
Human Cd3 Epsilon, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human cd3 epsilon/product/Sino Biological
Average 94 stars, based on 1 article reviews
human cd3 epsilon - by Bioz Stars, 2026-02
94/100 stars
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antigens human cd3  (Sino Biological)
94
Sino Biological antigens human cd3
KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with <t>CD3</t> bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.
Antigens Human Cd3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antigens human cd3/product/Sino Biological
Average 94 stars, based on 1 article reviews
antigens human cd3 - by Bioz Stars, 2026-02
94/100 stars
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cd3e fitc  (Sino Biological)
94
Sino Biological cd3e fitc
KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with <t>CD3</t> bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.
Cd3e Fitc, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd3e fitc/product/Sino Biological
Average 94 stars, based on 1 article reviews
cd3e fitc - by Bioz Stars, 2026-02
94/100 stars
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KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with CD3 bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.

Journal: Journal for Immunotherapy of Cancer

Article Title: Non-superagonist CD28-based dual-signal T cell engager targeting

doi: 10.1136/jitc-2025-013246

Figure Lengend Snippet: KK-LC-1×CD28 enhances the specific lysis of KK-LC-1-expressing target cells by T cells in vitro. ( A–C ) Dose-dependent lysis of MKN45, HGC27(oe-KK-LC-1), and HGC27 by unstimulated T cells varies proportionally with CD3 bispecific protein concentration (E:T ratio 10:1; incubation time, 48 hours). ( D ) Representative confocal microscopy images (upper) and surface projections (lower) of live/dead staining in MCSs. Viable cells (green: calcein AM) and dead cells (red: Propidium Iodide) are quantified; scale bar=100 µm. ( E ) Quantitative analysis of viability in MCSs. ( F ) Combination therapy (KK-LC-1×CD3 + KK-LC-1×CD28) induces cytotoxicity against MKN45 and HGC27(oe-KK-LC-1) cells across E:T ratios (1:1, 5:1, 10:1; 48 hours). KK-LC-1×CD3(2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). ( G ) The cytotoxic efficacy of KK-LC-1×CD3 monotherapy and combination therapy was evaluated under an E:T ratio of 10:1 for 48 hours, with or without T cell involvement. KK-LC-1×CD3 (2.5 µg/mL) KK-LC-1×CD28 (5 µg/mL). Data are represented as mean±SEM (n=3). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns: not significant. E:T, effector-to-target; KK-LC-1, Kita-Kyushu Lung Cancer Antigen-1; MCSs, multicellular spheroids.

Article Snippet: Similarly, the extracellular domains of human CD3 (Sino Biological, Cat# 10977-H08H) and CD28 (Sino Biological, Cat# 90182-C08H) were individually immobilized on CM5 chips via amine coupling under identical conditions.

Techniques: Lysis, Expressing, In Vitro, Protein Concentration, Incubation, Confocal Microscopy, Staining